黄立华,教授,博士生导师。2007年毕业于中国科学院动物研究所,获理学博士学位。2007至2009年在美国康乃尔大学做博士后研究。2009年10月引进到华南师范大学生命科学学院。长期从事昆虫生理和分子生物学研究。在Nature Ecology and Evolution、Cell Research、Insect Biochemistry and Molecular Biology、Insect Molecular Biology等国际刊物上发表论文30多篇。主编《分子生物学实验技术——基础与拓展》(2017,科学出版社)。
以斜纹夜蛾和家蚕为研究对象,通过比较基因组、转录组、蛋白质组等方法,结合CRISPR/Cas9、PiggyBac等基因组编辑和转基因技术开展如下研究:1)探讨昆虫变态发育的调控机理。重点解析热激蛋白对蜕皮激素在细胞内的信号传导调控作用;2)家蚕重要经济性状的基因鉴定及应用。重点鉴定吐丝、产卵、发育相关性状基因,解析其功能,并通过构建转基因家蚕以改良家蚕品种;3)转基因昆虫研究。综合应用多种基因编辑及转基因技术,构建转基因昆虫,以防治害虫。
(1)国家自然科学基金面上项目“家蚕“薄茧”突变体形成的分子机理研究”,2019年1月-2022年12月,主持人。
(2) 国家自然科学基金面上项目“热激蛋白70调控斜纹夜蛾蜕皮激素受体的分子机制”,2017年1月-2020年12月,主持人。
(3) 广州市科技计划项目:转基因家蚕体系的建立与应用,2015年-2018年,主持人。
(4)广东省自然科学基金:热激蛋白调控昆虫蜕皮激素受体(EcR)功能的分子机理2015年-2018年,主持人。
(5) 教育部留学回国基金“热激蛋白在蜕皮激素信号传导过程中的作用机理”。2012年,主持人。
(6) 国家自然科学基金面上项目“斜纹夜蛾变态发育期蜕皮激素受体(EcR/USP)互作蛋白的分离鉴定与功能分析”,2012年1月-2015年12月,主持人 。
(7) 国家自然科学(青年)基金“昆虫生长发育过程中热激蛋白基因与蜕皮激素的相互关系”, 2010年1月-2012年12月,主持人。
(8) 广东省高等学校人才引进专项基金 ,2010年1月-2012年12月,主持人。
1. Niu, K., Zhang, X.J., Deng H., Wu, F., Ren, Y., Xiang, H., Zheng, S.C., Liu, L., Huang, L.H., Zeng, B.J., Li, Sheng., Xia, Q.Y., Song, Q.S., Palli, S.R., Feng, Q.L.* 2018. BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori. Nucleic Acids Research, 46(4):1710-1723.
2. Feng, X.B., Zheng, Z.W., Zhang, X., Gu, J.,Feng, Q.L., Huang, L.H*. 2018. Discovering genes responsible for silk synthesis in Bombyx mori by piggyBac-based random insertional mutagenesis. Insect Science (IF: 2.026) Online.
3. Cheng, T.#, Wu, J. #, Wu, Y. #, Chilukuri, R,V. #, Huang, L. #, Yamamoto, K. #, Feng, L. #, Li, W. #, Chen, Z., Guo, H., Liu, J., Li, S., Wang, X., Peng, L., Liu, D., Guo, Y., Fu, B., Li, Z., Liu, C., Chen, Y., Tomar, A., Hilliou, F., Montagné, N., Jacquin-Joly, E., d'Alençon, E., Seth, R.K., Bhatnagar, R.K., Jouraku, A., Shiotsuki, T., Kadono-Okuda, K., Promboon, A., Smagghe, G., Arunkumar, K.P.*, Kishino, H. *, Goldsmith, M.R., Feng, Q. *, Xia, Q. *, Mita, K. * 2017.Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest. Nature Ecology and Evolution, 1(11): 1747-1756. (共同第一作者)
4. 黄立华,王亚琴,梁山,邓惠敏,谷峻,张晓娟. 2017. 分子生物学实验技术——基础与拓展. 北京:科学出版社.
5. 冯兴暴、黄立华、刘飞玲. 基于PiggyBac转座子系统构建构建随机突变体库的方法。专利号:201710522558.X
6. Hu, D., Luo, W., Fan, L.F., Liu, F.L., Gu, J., Deng, H.M., Zhang, C., Huang, L.H.*, Feng, Q.L. 2016. Dynamics and regulation of glycolysis-tricarboxylic acid metabolism in the midgut of Spodoptera litura during metamorphosis. Insect Molecular Biology, 25(2):153-162.
7. 范琳芳,何文婧,苏智仁,朱家明,罗威,张纯,黄立华*. 2016. 斜纹夜蛾酵母双杂交文库的构建及其在蜕皮激素受体互作蛋白筛选中的应用. 昆虫学报,59(8):801-811.
8. Zeng, B.J., Lv, Y., Zhang, L.L., Huang, L.H., Feng, Q.L. 2016. Cloning and structural characterization of juvenile hormone diol kinase in Spodoptera litura. Insect Science, 23:819-828.
9. Zhang, N., Liu, J., Chen,S.N., Huang, L.H., Feng, Q.L., Zheng, S.C.* 2016. Expression profiles of glutathione S-transferase superfamily in Spodoptera litura tolerated to sublethal doses of chlorpyrifos. Insect Science, 23: 675-687.
10. Tong, X.W., Chen, B., Huang, L.H.*, Feng, Q.L., Kang, L*. 2015. Proteomic analysis reveals that COP9 signalosome complex subunit 7A (CSN7A) is essential for the phase transition of migratory locust. Scientific Reports. 5:12542
11. Huang, L.X., Gong, Y.J.,Gu, J., Zeng, B.J., Huang, L.H.*, Feng, Q.L. 2015. Expression, subcellular localization and protein-protein interaction of four isoforms of EcR/USP in the common cutworm. Insect Science 22: 95-105
12. Shen, Y., Gong, Y.J., Gu, J., Huang, L.H.*, Feng. Q.L. 2014. Physiological effect of mild thermal stress and its induction of gene expression in the common cutworm, Spodoptera litura. J. Insect Physiol. 61: 34-41
13. Chen, W.J., Huang, L.X., Hu, D., Liu, L.Y., Gu, J., Huang, L.H.*, Feng, Q.L. 2014. Cloning, expression and chitin-binding activity of two peritrophin-like protein genes in the common cutworm, Spodoptera litura. Insect Science 21: 449-458.
14. Ou, J., Deng, H.M., Zheng, S.C., Huang, L.H., Feng, Q.L.*, Liu, L. * 2014. Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis. BMC Genomics, 15:820.
15. Gu, J., Huang, L.X., Gong, Y.J., Zheng, S.C., Liu, L., Huang, L.H*., Feng, Q.L. 2013. De novo characterization of transcriptome and gene expression dynamics in epidermis during the larval-pupal metamorphosis of common cutworm. Insect Biochemistry and Molecular Biology 43: 794-808
16. Gu, J., Huang, L.X., Shen, Y., Huang, L.H.*, Feng, Q.L. 2012. Hsp70 and small Hsps are the major heat shock protein members involved in midgut metamorphosis in the common cutworm, Spodoptera litura. Insect Molecular Biology 21(5): 535-543.
17. Deng, H.M., Zhang, J.L., Li, Y., Zheng, S.C., Liu, L., Huang, L.H., Xu, W.H., Pallic, S.R., Feng, Q.L*. 2012. Homeodomain POU and Abd-A proteins regulate the transcription of pupal genes during metamorphosis of the silkworm, Bombyx mori. PNAS 109: 12598-12603.
18. Zhang, X., Tiewsiri, K., Kain, W., Huang, L.H., Wang. P*. 2012. Resistance of Trichoplusia ni to Bacillus thuringiensis Toxin Cry1Ac Is Independent of Alteration of the Cadherin-Like Receptor for Cry Toxins. PLOS ONE 7(5): e35991
19. Rao, Z.C., He, W.Y., Liu, L., Zheng, S.C., Huang, L.H., Feng, Q.L. 2012. Identification, Expression and Target Gene Analyses of MicroRNAs in Spodoptera litura. PLOS ONE 7(5): e37730
20. Shen, Y., Gu, J., Huang, L.H*., Zheng, S.C., Liu, L., Xu, W.H., Feng, Q.L., Kang, L*. 2011. Cloning and expression analysis of six small heat shock protein genes in the common cutworm, Spodoptera litura. J Insect Physiol.57(7):1033-1044.
21. Terenius, O., Papanicolaou, A., Garbutt, J.S., et al., Huang, L.H., et al. RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design. 2010. Journal of Insect Physiology 57: 231-245.
22. Huang, L.H., Wang, C.Z., Kang, L. 2009. Cloning and expression of five heat shock protein genes in relation to cold hardening and development in the leafminer, Liriomyza sativa. Journal of Insect Physiology, 55: 279-285
23. Huang, L.H., Wang, H.S., Kang, L. 2008. Different evolutionary lineages of large and small heat shock proteins in Eukaryota. Cell Research, 18: 1074-1076
24. Huang, L.H., Kang, L. 2007. Cloning and inter-specific altered expression of heat shock protein genes in two leafminer species in response to thermal stress. Insect Molecular Biology, 16 (4): 491–500.(citation:117)
25. Huang, L.H., Chen, B., Kang, L. 2007. Impact of mild temperature hardening on thermotolerance, fecundity, and Hsp gene expression in Liriomyza huidobrensis. Journal of Insect Physiology, 53 (12): 1199-1205 (citation:92)
26. Wang, H.S., Wang, X.H., Zhou, C.S., Huang, L.H., Zhang, S.F., Guo, W., Kang, L. 2007. cDNA cloning of heat shock proteins and their expression in the two phases of the migratory locust. Insect Molecular Biology. 16: 207-219
地址:华南师范大学昆虫科学与技术研究所204
邮箱:huanglh@scnu.edu.cn